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, shown are superimposed buildings of NAD+ plus the pyridopyrimidine inhibitor within their respective LigA complexes.
Each individual phase in the ligation pathway relies upon on a unique subset on the LigA domains, with only the NTase area being essential for all steps. Area Ia is unique to NAD+-dependent ligases, is accountable for binding the NMN moiety of NAD+, and is needed for that response with NAD+ to kind the ligase-AMP intermediate (10, 11).
The motion entails a rigid overall body rotation in regards to the hinge section connecting the NTase and OB domains (indicated by the arrow
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